Electroporation is the application of an electric field that causes a brief breakdown of cell membranes to allow molecules to enter the cell.
A primary biomedical application of electroporation is the reintroduction of engineered T-cells for immunotherapy.
A Chimeric Antigen Receptor (CAR) is a receptor designed in the laboratory to bind to certain proteins on cancer cells. When introduced to a T-cell, they bind to the cancer cell such that the T-cell can attack the cancer. Electroporation is an effective means of re-introducing the CAR-T-cell to the body. In sufficient quantities, these CAR-T-cells will find and kill tumour cells.
Cells respond to electroporation depending on the cell diameter, DNA concentration, temperature, and the electroporation buffer.
Electroporation is optimized by altering the wave form, the field strength, the pulse length, the gap between the electrodes, and other parameters.
EHT BiPolar microsecond pulse generators (BMP) offer high voltage, square pulses with fast rise times, and are therefore ideally suited to electroporation.
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